| 质粒名称: | pEGFP-C1-EEEV nsP3 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002849 |
| 产品用途: | Mammalian expression of mEGFP-EEEV nsP3 |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
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| 载体名称: | pEGFP-C1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
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插入基因信息 |
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| 插入基因名称: | EEEV nsP3 |
| 物种来源: | Alphavirus |
| 密码子优化: | 大肠杆菌表达优化 |
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文献引用方法 |
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| 参考文献部分: |
Alphaviral non-structural protein-host RBP co-condensation as a mechanism to sustain virus replication
Molecular Cell, Volume 86, Issue 9, 2026
Yi Liu, Peiguo Yang
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pEGFP-C1-EEEV nsP3
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pEGFP-C1-EEEV nsP3载体质粒基本信息
pEGFP-C1-EEEV nsP3质粒图谱载体图谱和pEGFP-C1-EEEV nsP3载体序列质粒序列多克隆位点信息
pEGFP-C1-EEEV nsP3质粒载体简介
pEGFP-C1-EEEV nsP3质粒序列载体序列
LOCUS pEGFP-C1-EEEV nsP3 6358 bp DNA circular SYN 16-JUL-2026 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pEGFP-C1-EEEV nsP3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6358) AUTHORS 1243235 TITLE Direct Submission JOURNAL Exported Sep 7, 2026 from SnapGene 6.0.2 https://www.biofeng.com FEATURES Location/Qualifiers source 1..6358 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 304..507 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 552..1268 /codon_start=1 /product="enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1274..1298) /label=p61-1155 primer_bind 1284..1322 /label=p61-1148 CDS 1299..2957 /codon_start=1 /label=EEEV nsP3 /translation="APAYRVVRGDITKSNDEVIVNAANNKGQPGSGVCGALYRKWPGAF DKQPVATGKAHLVKHSPNVIHAVGPNFSRLSENEGDQKLSEVYMDIARIINNERFTKVS IPLLSTGIYAGGKDRVMQSLNHLFTAMDTTDADITIYCLDKQWESRIKEAITRKESVEE LTEDDRPVDIELVRVHPLSSLAGRPGYSTTEGKVYSYLEGTRFHQTAKDIAEIYAMWPN KQEANEQICLYVLGESMNSIRSKCPVEESEASSPPHTIPCLCNYAMTAERVYRLRMAKN EQFAVCSSFQLPKYRITGVQKIQCSKPVIFSGTVPPAIHPRKFASVTVEDTPVVQPERL VPRRPAPPVPVPARIPSPPCTSTNGSTTSIQSLGEDQSASASSGAEISVDQVSLWSIPS ATGFDVRTSSSLSLEQPTFPTMVVEAEIHASQGSLWSIPSITGSETRAPSPPSQDSRPS TPSASGSHTSVDLITFDSVAEILEDFSRSPFQFLSEIKPIPAPRTRVNNMSRSADTIKP IPKPRKCQVKYTQPPGVARVISAAEFDEFVRRHSN" primer_bind complement(2245..2257) /label=p61-1149 misc_feature 2274..2957 /label=HVD misc_feature 2274..2561 /label=Fragment A misc_feature 2562..2783 /label=Fragment B misc_feature 2709..2747 /label=G3BP binding motif misc_feature 2784..2954 /label=Fragment C misc_feature 2889..2939 /label=FXR binding motif primer_bind complement(2940..2972) /label=p61-1149 primer_bind 2958..2982 /label=p61-1154 polyA_signal 3087..3208 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(3215..3670) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3697..3801 /gene="bla" /label=AmpR promoter promoter 3803..4160 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4011..4146 /label=SV40 ori /note="SV40 origin of replication" CDS 4195..4989 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 5221..5268 /label=HSV TK poly(A) signal /note="herpesvirus thymidine kinase polyadenylation signal" rep_origin 5597..6185 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" enhancer join(6358,1..303) /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 481 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 541 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 601 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 661 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 721 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 781 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 841 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 901 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 961 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 1021 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 1081 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 1141 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg 1201 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 1261 tgtacaagtc cggactcaga tctcgagctc aagcttccgc acctgcatat cgcgtagtgc 1321 gaggtgacat taccaagagc aacgatgagg tcattgtgaa cgcagcaaac aataaaggac 1381 aacctggctc tggggtgtgc ggtgctctgt atcggaaatg gccaggggct tttgataaac 1441 aacctgtcgc caccggtaag gctcatctgg tgaaacacag ccctaacgtc attcacgccg 1501 tagggcccaa ttttagtcgg ctgtcagaaa atgaagggga tcaaaagctc tccgaagtgt 1561 acatggacat agcaagaata attaataatg agcggttcac caaagtcagc attcctctgc 1621 tgtccactgg catatatgct ggggggaagg acagggttat gcaaagtctg aaccacctct 1681 tcaccgcaat ggacacgaca gatgccgaca tcactattta ctgtttggat aaacaatggg 1741 agtctaggat taaggaagcc atcacacgca aggagtctgt ggaggaactg accgaggatg 1801 ataggccagt agacattgaa ctggtgcgcg tgcaccccct ctcttcactt gccggtagac 1861 ccgggtatag tactaccgag ggtaaggtct acagctacct ggagggtacc cgcttccatc 1921 aaaccgccaa ggatatcgcc gaaatttatg caatgtggcc aaataagcag gaggccaacg 1981 aacagatctg tctttacgta ctgggcgagt caatgaattc catccgcagc aaatgcccag 2041 tcgaagaatc tgaagcctcc tcccctcctc atacaatacc ctgtctgtgc aattacgcca 2101 tgaccgctga gcgcgtttat cggctgcgca tggccaagaa cgagcagttt gccgtatgta 2161 gcagtttcca gctgccaaag tatcgaatca caggcgtcca aaaaattcaa tgttcaaagc 2221 ctgttatttt ctctggaact gtcccgccgg caatccatcc cagaaagttt gcttcagtca 2281 cagttgagga tactcctgtt gtgcagccag agaggctcgt gcctcgcaga ccagctcccc 2341 cagttccagt tcccgctcga ataccttcac ccccatgcac ctccaccaac ggttccacga 2401 cctccatcca gtccctcggc gaggatcaga gcgccagtgc tagttccggg gctgaaatca 2461 gcgtggacca ggtgagcctg tggtctatac cctctgctac tggattcgac gtaagaacta 2521 gcagctcact ttccctcgaa cagcccactt tcccaacaat ggtcgtggag gccgaaattc 2581 acgccagcca gggctctctg tggtccattc ctagcattac aggatctgaa acgagggcac 2641 ccagcccacc ctcacaggat agtaggccat caacaccctc tgcaagcggt tcccatacat 2701 ccgtagattt gattaccttc gactccgtag ccgagattct ggaggacttt agcaggtctc 2761 cctttcaatt cctgtccgag atcaagccca tacccgcccc aaggacacgc gtgaataata 2821 tgtcacgcag cgccgataca attaaaccaa ttcccaagcc acgcaagtgt caggttaaat 2881 atacccaacc gcctggtgtt gctcgggtca tcagtgcggc cgaattcgac gagtttgtgc 2941 gccggcactc caattgagga tccaccggat ctagataact gatcataatc agccatacca 3001 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac 3061 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat 3121 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 3181 gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta 3241 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 3301 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg 3361 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 3421 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc 3481 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 3541 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 3601 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 3661 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 3721 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 3781 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt 3841 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 3901 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 3961 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 4021 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 4081 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 4141 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt 4201 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 4261 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 4321 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac 4381 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 4441 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 4501 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 4561 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 4621 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 4681 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag 4741 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 4801 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 4861 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 4921 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 4981 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat 5041 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 5101 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 5161 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga 5221 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg 5281 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg 5341 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg 5401 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga 5461 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 5521 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 5581 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 5641 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 5701 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 5761 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 5821 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 5881 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 5941 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 6001 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 6061 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 6121 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 6181 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 6241 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt 6301 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttcc //
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